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[DOI] [PMC free article] [PubMed] [Google Scholar] 660

Compared to FACS, our GST affinity cell sorting system does not require expensive equipment and is less likely to cause mechanical cell damage ( The recently reported positive cell sorting system utilizes GPI-anchored Twin-Strep-Tag as the affinity tag and allows the positive cells to be enriched with Strep-Tactin magnetic beads ( Reduced GSH is widely expressed in plant, animal, and fungal cells ( In brief, we develop a gene transfection-positive cell sorting system utilizing GPI-anchored GST as the affinity tag that enables positive cell isolation using GSH magnetic beads

10.1016/j.ejphar.2016.10.035 107 ZlatarM.KokotA.VuleticL

The redox-active sulphydryl group of GSH can protect cells from ROS by directly scavenging free radicals and acting as a cofactor for antioxidant enzymes such as glutathione peroxidases (Gpx1p, Gpx2p and Gpx3p)

Copper is a critical component involved in the formation of enzymes