Rats were run in two separate cohorts ( n = 56 per group/wave), where for each experimental test day rats received daily IP injections of vehicle, sCT (3 g/kg), liraglutide (50 g/kg), a combination of sCT and liraglutide (3 g/kg + 50 g/kg) or a stepwise administration of the amylin and GLP-1R analogs (rats received sCT treatment for the first week, liraglutide treatment for the second week, followed by a combination treatment of sCT + liraglutide daily for the remaining two weeks of testing)
Following treatment, 10M DCFHDA was added to each experimental group as per the manufacturers instructions
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