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Feb 2019;187(2):341-356

doi: 10.1093/annonc/mdh006 14 RehmanSKHaynesJCollignonEBrownKRWangYNixonAMLet al

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UAE Research suggests that senescent cells impair proteasome activity in the heart, leading to the accumulation of damaged proteins and reduced cardiac function

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In parallel, we cloned and purified 57 Cr GPDH 705 -FLAG variant with an additional 30 amino acids from the unstructured region at the N-terminus to eliminate the possibility that the GPP domain in the construct we used in our initial cellular studies was truncated due to potentially incorrectly predicted boundaries of the chloroplast targeting sequence (CTS) ( Figure 2a , Supplementary Figure S1 , Supplementary Figure S2b , Supplementary Figure S4 )