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[4] Cell Line:PC12 cells Concentration:4 mM Incubation Time:2 h pretreatment + 12 h cadmium exposure Result:Significantly reduced the number of TUNEL-positive PC12 cells

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The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation