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Before use, the protein was denatured in 6 M urea and supplemented with 2 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, Solna, Sweden) at pH 7.4 to obtain complete reduction and dissolution of potential aggregates

Intensive management of obesity in people with Prader-Willi syndrome

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Am J Nephrol 2017;46:213-221

However, responsible access to these drugs is paramount, said New York-based obesity-medicine specialist Dr