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Briefly, sitting drop crystallisations were set up in 96-well trays using a Cartesian robot with a reservoir solution of 1.5 M ammonium sulfate and 0.1 M Tris at pH 8.5, each crystallisation drop containing 100 nL each of protein and reservoir solutions

In contrast, the MAP3K17/18MKK3MPK1/2/7/14 cascades operate downstream of SnRK2 in response to ABA, independently of Ca 2 signaling (Yang et al

Suggested use

Synthetic gene sequences and primers for the mutations were custom synthesized by Integrated DNA Technologies (IDT) (Supplementary Table 3)