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M1, M3 and M5 couple with G q , and when stimulated mediate the activation of phospholipase C resulting in an increase in [Ca 2+ ] i and protein kinase C activation

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J Steroid Biochem Mol Biol 155:2634 Gourlaouen M, Welti JC, Vasudev NS, Reynolds AR (2013) Essential role for endocytosis in the growth factor-stimulated activation of ERK1/2 in endothelial cells

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Other parameters like growth velocity (in children), body composition, bone density, or general well-being can help assess effectiveness

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