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After three washes with PBS, the cells were incubated with an Oil red O solution (Sigma-Aldrich) for 10 min

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Research shows GHK-Cu's therapeutic dose is approximately 300-fold below its toxic threshold

Iron affects the homeostasis of bile flow and bile components by altering the activity of hepatic enzymes that regulate cholesterol and bile salt levels [22]

Furthermore, these studies were rather small-scaled with short duration and design flaws were apparent