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Accordingly, in a recent genome-wide CRISPR-screening, it was reported that the inactivation of the gene coding for the ABC-transporter ABCC1, which pumps GSH-conjugates out of the cell, favours cell growth in the presence of FA 44

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Precise control of operational parameterssuch as power, temperature, and extraction durationis essential, as excessive energy input or prolonged treatment can degrade sensitive polyphenolic structures, potentially lowering yield or altering compound profiles ( 3.1.4 Supercritical fluid extraction method Supercritical fluid extraction is an alternative two-step technique