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Shaking creates strong mechanical forces that can physically damage or denature the delicate peptide structures

Inhibition of the target gene expression can occur through various mechanisms such as RNA interference, once the sense-antisense mRNA duplex has formed, transcriptional interference, in which the NAT can act as a physical barrier for RNA polymerase activity, or epigenetic methylation of the sense gene DNA, thus inhibiting the transcription of the sense mRNA transcript [55, 56]

11 have shown the efficacy of NACA against L-buthionine-(S,R) sulfoximine (BSO, a glutathione synthesis inhibitor)- induced cataract in wistar rat pups