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GLP-1 (Glucagon-Like Peptide-1) is a hormone naturally produced in your intestines after eating

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RNA isolation and reverse transcription polymerase chain reaction analysis In the pituitary, we quantified gene expression of (1) the ACTH precursor POMC, (2) the main processing enzymes cleaving POMC into smaller fragments: proprotein convertase 1 (PC1/3) and proprotein convertase 2 (PC2), (3) the transcriptional regulators hereof, which are themselves regulated at the mRNA levels by CRH, AVP and/or a GR ligand (CRH receptor (CRH-R), AVP receptor (AVP-R), GR, Nur77 and Tpit), (4) a GR-ligand-binding-stimulated inhibitor of CRH-driven secretion of mature ACTH, Annexin A1, and (5) pro-inflammatory cytokines (tumor necrosis factor alpha (TNF-) and leukemia inhibitory factor (LIF))