Oxidation of disulfide bonds of Pt GSTF8 Disulfide bonds between C13 of purified apo- Pt GSTF8 variants and GSH were formed by incubating 20 M Pt GSTF8 with 0 mM or 1 mM GSSG in the presence of 200 M GSH in 10 mM MOPSO, pH 6.45 for 2 h at 30 C, 800 rpm using a 3 mm shaking diameter
This isn't community vetting
Not sure if you know this but it's worth mentioning that if you have any neurological symptoms when you were diagnosed, the B12 regime (according to all guidelines) is six loading doses of 1mg B12 and then 1mg injections every other day until no further improvement (for some people this takes many months)
Navas, R
DSIP crosses the blood-brain barrier freely an unusual property for a peptide of its size via a non-competitive, non-saturable passive mechanism whose physicochemical determinants (lipophilicity, charge, molecular weight, and protein-binding) are now well characterised
In some embodiments, the deliverable osmolarity of any formulation described herein is designed to be isotonic with the targeted otic structure (e.g., endolymph, perilymph, or the like)