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Generally, biologic activity of ATRA such as gene expression or inhibition through direct DNA interaction is being exerted by binding to nuclear retinoid receptors (ligand-activated transcription factors) like (all- trans ) retinoic acid receptors (RARs) or retinoid X receptors (RXRs) which together build a heterodimer [83, 96,97,98,99]

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LeCun, Y., Bengio, Y

Jones, Glutathione in Human Plasma: Decline in Association with Aging, Age-Related Macular Degeneration, and Diabetes

Adding higher or lower concentration of standard may cause inconsistent signal or false positive