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The proteins were transferred from the gel to a nitrocellulose membrane using the TransBlot Semi-Dry Electrophoretic Transfer Cell (Bio-Rad , Hercules, CA, USA) and blocked with 5% skim milk in Tween-Tris-buffered saline (TTBS

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Normal values vary based on age and sex: Ages 0-10: 14-495 ng/mL Ages 11-17: 17-633 ng/mL Ages 18-55: 37-245 ng/mL Ages 55+: 25-232 ng/mL Summary: Doctors typically check IGF-1 status when they suspect growth abnormalities, pituitary dysfunction or malnutrition