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At the same time, the authors also found that GSH content in 4T1 cells was sharply cut down owing to Glud-MFo-c GSH-depletion ability (Figure 6C), which effectively amplified Ce6-produced 1 O 2 accumulation, and provided a potential method to combat cancer

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2.3 Preparation of Pc GKB4 inoculum The Pc GKB4 stock culture was sub-cultured onto PDA plates (19.5 g/L potato dextrose agar and 7.5 g/L agar) and incubated in the dark at 22C for 4 days

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Cells were washed with PBS (Sigma-Aldrich) and treated with chlorophyllin (50 M), docetaxel (31.45 M), and their combination in RPMI-1640 containing 2% FBS