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The antioxidant activity was expressed as the antioxidant activity index (AAI), calculated using the following formula: AAI = Final concentration of DPPH (g/mL)/IC 50 (g/mL) This approach allows the AAI to be calculated based on the mass of DPPH and the mass of the tested compound in the reaction, producing a constant value for each compound that is independent of the DPPH and sample concentrations used

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10.1093/toxsci/kfq039