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As emulsifier, commonly utilized and widespread pharmaceutical excipient, PEG-35 castor oil (PEG-35 CO), was combined with the lipid phase GMCC/C8 1:4 (m/m) or RM in a 1:1 (m/m) ratio in order to obtain sufficient self-emulsification

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Finally, 50 L suspension buffer was used to re-suspend and obtain complete and unbroken nuclei

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Preller KH, Burt JB, Ji JL, Schleifer CH, Adkinson BD, Stmpfli P, et al