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Am J Clin Nutr 62:412416 Mao S, Zhang A, Huang S (2014) Meta-analysis of Zn, Cu and Fe in the hair of Chinese children with recurrent respiratory tract infection

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The blood was obtained from the heart and the serum was isolated by allowing the blood to coagulate, followed by centrifugation at 1600 g for 10 min, ensuring the separation of serum from other blood components for subsequent evaluations