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Cells were harvested using 110 L ice cold lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl 2 , 1% (v/v) Triton X-100 and 10% (v/v) of protease and phosphatase inhibitor cocktail (Roche)
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What symptoms does the article say should prompt a call to the prescriber rather than a schedule tweak
Human data are often associative rather than interventional, assays lack standardization, and receptor mechanisms may be incomplete or contested
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