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In animal models of atopic dermatitis-like inflammation, topical GHK-Cu application results in 45% reduction in inflammation scores and 60% improvement in barrier function measures

Plasma free fatty acid (FFA) concentrations were measured using the Free Fatty Acid Quantification Kit (Sigma-Aldrich) in separate cohorts of mice, including both sexes of whole-body Gpr146/ and Gpr146/ mice on HFD, as well as adipose-specific and liver-specific knockout models following HFD feeding

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Maintenance protocols typically use lower doses than initial loading phases

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