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Gromadzka G, Czerwiska J, Krzemiska E, et al (2024) Wilson's disease-crossroads of genetics, inflammation and immunity/autoimmunity: clinical and molecular issues

Jeffrey I

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Dissolving 0.33g of Fmoc-His-Aib-OSu crude product and 1g of Boc protected Arg34GLP-1 (9-37) in 20mL of N, N-dimethylformamide, adding 2mL of N, N-diisopropylethylamine, reacting at room temperature for 8h, adding 20mL of mixed solution of methyl tert-ether and petroleum ether, precipitating and centrifuging to obtain Fmoc-GLP-1 (Lys-Lys) 20 Boc), the crude product was added to a trifluoroacetic acid solution, stirred at low temperature for 2 hours, 400mL of methyl-tert-ether was added, centrifugation was performed to obtain 0.98g of a solid, the crude product was dissolved in 20mL of N, N-dimethylformamide and 2mL of N, N-diisopropylethylamine was added, 0.33g of the side chain of compound 1 was dissolved in 2mL of N, N-dimethylformamide, slowly added dropwise to the reaction solution, reacted at room temperature for 2 to 3 hours, added to 400mL of a mixed solution of methyl-tert-ether and petroleum ether, centrifuged to obtain a solid, the above solid was added to 4mL of a 20% piperidine DMF solution, reacted at room temperature for 1 hour, and 40mL of a mixed solution of methyl-tert-ether and petroleum ether was added, centrifuged to obtain a solid, the above solid was added to 10mL of a cleavage solution (TFA: TIS: DCM =95 2), reacted at room temperature for 2 to 4 hours, 100mL of a mixed solution of methyl-tert-ether and petroleum ether was added, centrifugation to obtain 0.8g of a solid having a purity of57.15%, the specific liquid phase map is shown in FIG

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