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Samples and standards were derivatized with 30 l of isobutyl-hydroxylamine in pyridine for 20 min at 80C and with 30 l of N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA) for 60 min at 80C before they were transferred to GC-MS vials

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Shortly, two methods were applied for separate extraction of lipids and polar/semipolar metabolites and the extracts were then analyzed using an ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOFMS) as described previously [39] and the data were processed using MZmine 2.53 [45]

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The team discussed how this technology could expand Setria's applications beyond traditional supplement formats, potentially enabling new beverage products and acute-use applications that weren't feasible before