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While carbonic anhydrases generally facilitate the interconversion of CO 2 and HCO 3 , CA9 is a membrane-bound isoenzyme traditionally associated with pH homeostasis in tumor niches ( 2 Materials and methods 2.1 Animals Procured from Charles River (Beijing, China), C57BL/6 male mice (1822 g body weight) at 68 weeks of age were utilized for this study