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However, their use is limited by side effects (drowsiness, constipation), risk of dependency, and individual variability in drug response

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We should know the side effects that can be caused in the long term so that patients can be counseled on how to avoid long-term issues. Kipnis said he would like to see better long-term data as the next steps in this research

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Researchers block free GSH using alkylating agents such as 2-vinylpyridine (2-VP) or N-ethylmaleimide (NEM) before running the assay, which isolates GSSG for independent measurement

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