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glutamicum for the screening of carotenoid production were grown in CGXII minimal medium 67 or in CGXII minimal media with optimized trace salts concentrations (25 g L 1 FeSO 4 7H 2 O, 2.5 g L 1 MnSO 4 x H 2 O, 1.18 g L 1 ZnSO 4 7H 2 O, 0.15 g L 1 CuSO 4 5H 2 O, 0.015 g L 1 NiCl 2 6H 2 O) 52 , called CGXII opt , both supplemented with 40 g L 1 glucose and supplemented with 1 mM IPTG for induction if needed after washing in the minimal medium

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