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Epitomics), The membranes were washed three times in TBST for 5 mins each and were incubated at room temperature for 1 hr with horseradish peroxidase conjugated rabbit anti-mouse or anti-rabbit IgG secondary antibody (Sigma-Aldrich, anti-mouse Cat

The primary published evidence base includes the Phase 2 randomised controlled trial reported by Jastreboff et al

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Three sessions per week at high intensity preserves more muscle than five sessions at moderate intensity