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Dynabeads Protein G (Dynal Biotech: Invitrogen) equilibrated in Co-IP buffer was added to the mixture, which was gently mixed for another 1 h

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The expression of AR in the HF-KCs has long been debated, Asada et al found that AR mRNA was expressed not only in the dermal papilla and dermal sheath compartments but also in the ORS regions of human scalp hair follicles[44]