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For cloning purposes, cells were grown in lysogeny broth (LB) medium (10 g L 1 tryptone, 5 g L 1 yeast extract, and 10 g L 1 sodium chloride) at 37C on a shaking incubator (shaking frequency: 180 min 1 , eccentricity: 25 mm)

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Kowloon, he wrote, had become a cesspool of iniquity, with heroin divans, brothels and everything unsavoury. The Chinese claims to sovereignty over Kowloon did not extend to any day-to-day administration