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Efficacy of oral cobalamin (vitamin B12) therapy

DMEM/F12 medium (supplemented with fetal bovine serum (20%) and penicillin/streptomycin (1% v/v) (both from Gibco by Life Technologies, Paisley, Scotland, UK) was utilized for culturing isolated MSCs

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A pivotal study (Yano et al., Cell, 2015) showed that the microbiota influence the serotonergic system through an entire constellation of metabolites many produced by spore-forming Clostridia, the same group known for butyrate and broad gut-supportive functions