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Second, the billing-complaint pattern is consistent enough across multiple independent BBB filings to suggest a systemic process gap rather than isolated incidents

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For cFos studies, two to six sections per mouse at the level of the hypothalamus (bregma 0.46 to 1.06 mm) and hindbrain (bregma 7.2 to 7.76 mm) were counted bilaterally and averaged across sections for each mouse

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These important interactions are beyond the scope of this initial investigation, but will be incorporated in future studies