The plates were sealed with AeraSeal films (Merck, A9224) to allow gas exchange in cultures and incubated at 30 C, 225 rpm and 80% relative humidity in a humidity shaker (Minitron, INFORS)
The initial elution was isocratic with 30% acetonitrile for 2 min, followed by a linear gradient for 15 min up to 100% acetonitrile
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