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The HIF1-dependent cell reprogramming induces KG production, ICT, and 2HG synthesis by the wild-type IDH enzymes [84], inhibition of the alpha-ketoglutarate dehydrogenase (KGDH) converting KG to succinyl-CoA [83], and expression of Gln transporters such as SLC38A2 [86] and SLC38A1 [87]

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Step 3: Draw your calculated volume of BAC water Using the larger syringe (23ml), draw an amount of air equal to your calculated BAC water volume

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