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Figure 6 13 Future research directions and emerging prospects: the estrogen-gut-brain axis The GM and bone relationship niche is vast for precision medicine

After three washes with free-serum media, cells were collected, counted, and analyzed for their fluorescence intensity using a fluorescence microplate reader (Thermo Fisher Scientific, Waltham, MA, USA) with its excitation and emission wavelengths at 488 nm and 525 nm [29, 30]

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