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The solvent was evaporated in vacuo to give a crude mixture that was further purified by HPLC (water and acetonitrile, both supplemented with 0.1% trifluoroacetic acid) on a Cosmosil C18 AR-II column (5.0 m, 10 250 mm
Am J Physiol Endocrinol Metab 290(6):E1118-1123
The raw material is characterized by high purity
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