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In the first step, we coupled biotin-BiLockCatcher with an excess of the first antibody (usually 1.5 molar equivalents) to ensure complete coupling of the SpyCatcher003 within one to three hours

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For instance, the acylation reaction using L-carnitine and propionyl chloride can be carried out under conditions using dry propionic acid as the solvent and p-toluenesulfonic acid as the catalyst