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One hundred nanograms of tRNA fraction or purified tRNA were digested with 0.5 U nuclease P1 (US Biological) and 0.1 U phosphodiesterase I (Sigma) in 22 l reactions containing 50 mM TrisHCl (pH 5.3) and 10 mM ZnCl 2 at 37 C for 1 h

Heat exposure above 30 degrees Celsius Temperatures at or above 30 degrees Celsius (86 degrees Fahrenheit) cause rapid, often irreversible degradation

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