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Briefly, the frozen cortical samples were homogenized in SDT-lysis buffer (100 mM TrisHCl (pH 7.6) containing 0.1 M DTT) using an ultrasonic homogenizer, and the homogenates were heated at 95 C for 3 min

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The toxicologically considerable changes in mean body weight were not observed in the animals treated with T1 (Supplementary Table S2 and Fig

Alcohol is not automatically prohibited, but caution is warranted