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Although vomiting was persistent, the semaglutide treatment was continued because a reduction in body weight was achieved (BMI: 42.2 kg/m 2 )

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It is an 83-amino-acid peptide bearing an arginine substitution at position 3 and a 13-amino-acid extension at the N-terminus

The next day, cell mixtures were scraped and resuspended in 1 ml LB and 1000X dilutions of this conjugation mixture were plated on 10 large (150 mm 15 mm) LB plates (lacking DAP) supplemented with 50 g/ml kanamycin

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