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The very first amino acid in a peptide chain will lose its -COOH (carboxyl) group to form a covalent bond with the second amino acid in the chain, but its -NH 2 (amino) group will be intact

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EAE is predicated on a primary anti-myelin T cell response and therefore disease is most commonly induced by inoculating mice with myelin peptides together with adjuvant to stimulate an immune reaction against these extrinsically-supplied myelin antigens (Robinson et al., 2014)

doi: 10.1007/BF02253527