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After a 48-hour incubation with the drugs, the medium was replaced with Seahorse XF base medium (Agilent Technologies, USA) and incubated for 1 h at 37 C in a CO 2 -free environment

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Lipid peroxidation through accumulation of malondialdehyde (MDA) was determined by homogenizing the tissue in ice-cold mixture of 20% (v/v) TCA containing 0.5% thio barbituric acid (TBA) (1:1)