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After ultracentrifugation at 200,000 g for 20 min at 4 C the resulting supernatants were used in the assays

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doi: 10.1007/s00266-005-0210-1

Furthermore, H&E staining showed that compared to the aorta of normal aging mice, the aorta of mice treated with FOXO4-DRI was thinner, and the PWV value was lower in the ultrasound detection results, which means that the mice treated with FOXO4-DRI had better vascular elasticity and better vascular function than normal aging mice (Figures 1D,E)

Modak S, Singh H, Singh RP, Mukhopadhyay A (2017) Platinum based chemotherapy induced fatigue among cancer patients

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