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[1] [2] The standard initiation regimen begins with 0.25 mg once weekly for four weeks, which serves primarily as a dose escalation phase to improve gastrointestinal tolerability rather than to achieve glycaemic control

Nygren et al

Mechanism of Action: The Highway and Traffic Paradigm

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While the location itself does not change effectiveness, repeatedly injecting into the exact same spot can lead to irritation or complications such as lipodystrophy, where the fat under the skin changes in a way that may affect absorption

The recycling step from GSSG back to GSH depends on an enzyme named glutathione reductase , which requires NADPH to work