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After you insert, pull back slightly on the plunger of the syringe

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Animals were anesthetized intramuscularly with a ketaminexylazine mixture composed of 30 mg/kg ketamine and 12 mg/kg xylazine, respectively, with a total injection volume of 1.2 mL/kg

Using the wrong solution type is another frequent error: substituting tap water, saline, or an unverified liquid for pharmaceutical-grade bacteriostatic or sterile water introduces contamination risk regardless of how carefully everything else is handled