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The reason has been ascribed to loss of proteasome activity over time

The metabolic profile of tumors depends on both the responsible genetic lesion and tissue type

Older adults, those with liver disease, alcohol use, malnutrition, CYP2E1 genetic variations, and users of other hepatotoxic drugs face higher depletion risk

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Freezing reconstituted peptides causes ice crystal formation, which physically disrupts the tertiary structure of the folded peptide