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cDNA was generated from 2 g isolated RNA using M-MLV reverse transcriptase (Promega) and diluted 1:15 for use in 12 L qPCR reactions run on the StepOnePlus system (Applied Biosystem)

2) The findings of the study performed by research Cangul et al consistently demonstrated that topical application of TCC significantly enhances open-wound healing in rabbits compared to zinc oxide or no treatment, particularly in the early stages of the healing process

(2018) provided a comprehensive review of this angiogenic profile, linking it to the peptides broader growth factor modulation effects

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