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Western blot results revealed that, in si-NC cells, Erastin robustly downregulated the expression of tight junction proteins Claudin-1 and Occludin, as well as anti-ferroptotic markers GPX4 and SLC7A11 and these protein deficits were largely restored upon ATT-I co-treatment

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Because vitamin B12 is water-soluble, excesses will simply leave the body in urine

Aliquoting maintains consistent peptide concentration across experiments

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Sanitize the Injection Site Clean the chosen area with a fresh alcohol swab and let it dry completely