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this impairment seems to prevalently involve the autonomic nervous system and not related to a high alcohol intake

For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)

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GHK-Cu (Copper Tripeptide-1) Regeneration signal

These changes may lead to a satiety hormone imbalance and signal impairments in the hypothalamus, which are involved in satiety (133)

Clinical testing is appropriate for documented medical indications