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Cells were directly (for fresh hepatocytes) or after thawing (for cryopreserved hepatocytes) suspended at a concentration of 1 x 10 6 cells/ml in a seeding medium composed of Williams E medium, supplemented with 10% (vol/vol) FCS, 5 g/ml bovine insulin, 100 IU/ml penicillin, 100 g/ml streptomycin, and 2 mM glutamine