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Increases in expression of the immune checkpoint gene CTLA4 and immunoregulatory gene PTPN2 have been observed in patient skin alongside increases in COMT (Tanwar et al., 2022)

The Antioxidant Role of Glutathione and N-Acetyl-Cysteine Supplements and Exercise-Induced Oxidative Stress.J Int Soc Sports Nutr .2005

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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]