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Mouse pancreatic islet isolation and culture Islets were isolated from mice pancreata previously infused with RPMI-1640 medium, (21875091, Thermo Fisher Scientific) containing 1 mg/mL collagenase ( Clostridium histolyticum ) (S1745602, Nordmark Biochemicals), dissected, and incubated in a 37C water bath for 10 minutes to digest the tissue

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Extra- and intracellular extracts and their respective blanks, RPMI media or lysis buffer, were loaded onto a 96-well plate and absorbance was measured at 405 nm using a Multiskan FC Microplate Photometer (Thermo Fisher Scientific, USA)

Endoplasmic reticulum stress and the inflammatory basis of metabolic disease